In the surgical treatment of tuberculosis of the bones, excision of the lesion site leaves defects in the bone structure. Recent research has shown benefits for bone tissue support, such as tricalcium phosphate, as regrowth materials. These biocompatible engineering materials have good bone inductivity and biologic mechanical performance. The goal of this study was to evaluate the use of 3D printing, a new technology, to design and build 3-dimensional support structures for use in grafting at lesion sites and for use in embedding the sustained release anti-tuberculosis drugs Rifampin and Isoniazid and determine the in vivo performance of these structures. In addition to mechanical studies, osteogenesis, cell viability, and migration were all observed, using Wistar rat models, to determine the effectiveness of this material as a biological support. The bone support showed good resistance to compression, similar to the spongiest bone tissue, and high porosity. In vivo studies showed that the material had a stable time release of Rifampin and Isoniazid through 90 days and achieved effective killing of the tuberculosis-causing bacteria. Finally, the support allowed for good migration and survival of rat bone marrow mesenchymal stem cells, leading to successful bone regrowth and repair. These results imply that the use of 3D printing of tricalcium phosphate scaffolds for bone excision repair and time-release treatment of tuberculosis shows great promise for future treatment of patients with tuberculosis of the bones.