3D Bioplotter Research Papers

Displaying all papers about Thrombin (1 results)

Bioprinted fibrin-factor XIII-hyaluronate hydrogel scaffolds with encapsulated Schwann cells and their in vitro characterization for use in nerve regeneration

Bioprinting 2016 Volume 5, March 2017, Pages 1-9

The blood clotting protein fibrin contains cell-binding domains, providing potential advantage for the fabrication of tissue repair scaffolds and for live cell encapsulation. However, fabrication of fibrin scaffolds with encapsulated cells using three dimensional (3D) printing has proven challenging due to the mechanical difficulties of fabricating protein hydrogel scaffolds with defined microstructure. For example, extrusion based 3D printing of fibrin is generally unfeasible because of the low viscosity of precursor fibrinogen solution. Here we describe a novel technique for bioprinting of fibrin scaffolds by extruding fibrinogen solution into thrombin solution, utilizing hyaluronic acid (HA) and polyvinyl alcohol (PVA) to increase…