3D Bioplotter Research Papers

Displaying all papers about Electrospinning (7 results)

Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications

BioMed Research International 2017 Volume 2017, Article ID 6956794

Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally controlled fabrication of three-dimensional single/multimaterial constructs with precisely ordered fiber and pore architecture in a single build. However, this approach generally lacks the ability to achieve submicron resolution features to mimic native tissue. The goal of this study was to fabricate and evaluate 3D printed, electrospun, and combination of 3D printed/electrospun scaffolds to mimic the native architecture of heterogeneous tissue. We assessed their…

Multiscale fabrication of biomimetic scaffolds for tympanic membrane tissue engineering

Biofabrication 2015 Volume 7, Number 2, Article 025005

The tympanic membrane (TM) is a thin tissue able to efficiently collect and transmit sound vibrations across the middle ear thanks to the particular orientation of its collagen fibers, radiate on one side and circular on the opposite side. Through the combination of advanced scaffolds and autologous cells, tissue engineering (TE) could offer valuable alternatives to autografting in major TM lesions. In this study, a multiscale approach based on electrospinning (ES) and additive manufacturing (AM) was investigated to fabricate scaffolds, based on FDA approved copolymers, resembling the anatomic features and collagen fiber arrangement of the human TM. A single scale…

Combining technologies to create bioactive hybrid scaffolds for bone tissue engineering

Biomatter 2013 Volume 3, Issue 2, e23705

Combining technologies to engineer scaffolds that can offer physical and chemical cues to cells is an attractive approach in tissue engineering and regenerative medicine. In this study, we have fabricated polymer-ceramic hybrid scaffolds for bone regeneration by combining rapid prototyping (RP), electrospinning (ESP) and a biomimetic coating method in order to provide mechanical support and a physico-chemical environment mimicking both the organic and inorganic phases of bone extracellular matrix (ECM). Poly(ethylene oxide terephthalate)-poly(buthylene terephthalate) (PEOT/PBT) block copolymer was used to produce three dimensional scaffolds by combining 3D fiber (3DF) deposition, and ESP, and these constructs were then coated with a…

Layered Gradient Nonwovens of In Situ Crosslinked Electrospun Collagenous Nanofibers Used as Modular Scaffold Systems for Soft Tissue Regeneration

Advanced Functional Materials 2013 Volume 23, Issue 26, Pages 3277-3285

In a versatile modular scaffold system, gradient nonwovens of in situ crosslinked gelatin nanofibers (CGN), fabricated by reactive electrospinning, are laminated with perforated layers and nonwovens of thermoplastic non-crosslinked biodegradable polyesters. The addition of glyoxal to a gelatin solution in a non-toxic solvent mixture consisting of acetic acid, ethyl acetate, and water (5:3:2 w/w/w) enables the in situ crosslinking of gelatin nanofibers during electrospinning. The use of this fluorine-free crosslinking system eliminates the need of post-treatment crosslinking and purification steps typical for conventional CGN scaffolds. The slowly progressing crosslinking of the dissolved gelatin in the presence of glyoxal increases the…

Hierarchical starch‐based fibrous scaffold for bone tissue engineering applications

Journal of Tissue Engineering and Regenerative Medicine 2009 Volume 3, Issue 1, Pages 37-42

Fibrous structures mimicking the morphology of the natural extracellular matrix are considered promising scaffolds for tissue engineering. This work aims to develop a novel hierarchical starch-based scaffold. Such scaffolds were obtained by a combination of starch-polycaprolactone micro- and polycaprolactone nano-motifs, respectively produced by rapid prototyping (RP) and electrospinning techniques. Scanning electron microscopy (SEM) and micro-computed tomography analysis showed the successful fabrication of a multilayer scaffold composed of parallel aligned microfibres in a grid-like arrangement, intercalated by a mesh-like structure with randomly distributed nanofibres (NFM). Human osteoblast-like cells were dynamically seeded on the scaffolds, using spinner flasks, and cultured for 7…

Hybrid Process for Fabricating 3D Hierarchical Scaffolds Combining Rapid Prototyping and Electrospinning

Macromolecular Rapid Communications 2008 Volume29, Issue19, Pages 1577-1581

An ideal scaffold should have good mechanical properties and provide a biologically functional implant site. A rapid prototyping system has been introduced as a good method of fabricating 3D scaffolds that mimic the structure in the human body. However, the scaffolds have strands that are too smooth and a pore size that is too large relative to the seeded cells and present unfavorable conditions for initial cell attachment. To overcome these problems, we propose a hybrid technology combining a 3D rapid prototyping system and an electrospinning process to produce a hierarchical 3D biomedical scaffold. The resulting structure consists of alternating…

3D Fiber-Deposited Electrospun Integrated Scaffolds Enhance Cartilage Tissue Formation

Advanced Functional Materials 2008 Volume 18, Issue 1, Pages 53-60

Despite the periodical and completely interconnected pore network that characterizes rapid prototyped scaffolds, cell seeding efficiency remains still a critical factor for optimal tissue regeneration. This can be mainly attributed to the current resolution limits in pore size. We present here novel three-dimensional (3D) scaffolds fabricated by combining 3D fiber deposition (3DF) and electrospinning (ESP). Scaffolds consisted of integrated 3DF periodical macrofiber and random ESP microfiber networks (3DFESP). The 3DF scaffold provides structural integrity and mechanical properties, while the ESP network works as a “sieving” and cell entrapment system and offers?at the same time?cues at the extracellular matrix (ECM) scale….