3D Bioplotter Research Papers

Displaying all papers by N. Eliopoulos (1 results)

Design and Fabrication of 3D Porous Scaffolds to Facilitate Cell-Based Gene Therapy

Tissue Engineering Part A 2008 Volume: 14 Issue 6, Pages 1037-1048

Biomaterials capable of efficient gene delivery by embedded cells provide a fundamental tool for the treatment of acquired or hereditary diseases. A major obstacle is maintaining adequate nutrient and oxygen diffusion to cells within the biomaterial. In this study, we combined the solid free-form fabrication and porogen leaching techniques to fabricate three-dimensional scaffolds, with bimodal pore size distribution, for cell-based gene delivery. The objective of this study was to design micro-/macroporous scaffolds to improve cell viability and drug delivery. Murine bone marrow-derived mesenchymal stromal cells (MSCs) genetically engineered to secrete erythropoietin (EPO) were seeded onto poly-l-lactide (PLLA) scaffolds with different…