3D Bioplotter Research Papers

Displaying all papers by J. M. Oliveira (6 results)

Entrapped in cage (EiC) scaffolds of 3D-printed polycaprolactone and porous silk fibroin for meniscus tissue engineering

Biofabrication 2020 Volume 12, Number 2, Article 025028

The meniscus has critical functions in the knee joint kinematics and homeostasis. Injuries of the meniscus are frequent, and the lack of a functional meniscus between the femur and tibial plateau can cause articular cartilage degeneration leading to osteoarthritis development and progression. Regeneration of meniscus tissue has outstanding challenges to be addressed. In the current study, novel Entrapped in cage (EiC) scaffolds of 3D-printed polycaprolactone (PCL) and porous silk fibroin were proposed for meniscus tissue engineering. As confirmed by micro-structural analysis the entrapment of silk fibroin was successful, and all scaffolds had excellent interconnectivity (≥99%). The EiC scaffolds had more…

Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation

Journal of Materials Science: Materials in Medicine 2019 Volume 30, Article 63

The menisci have crucial roles in the knee, chondroprotection being the primary. Meniscus repair or substitution is favored in the clinical management of the meniscus lesions with given indications. The outstanding challenges with the meniscal scaffolds include the required biomechanical behavior and features. Suturability is one of the prerequisites for both implantation and implant survival. Therefore, we proposed herein a novel highly interconnected suturable porous scaffolds from regenerated silk fibroin that is reinforced with 3D-printed polycaprolactone (PCL) mesh in the middle, on the transverse plane to enhance the suture-holding capacity. Results showed that the reinforcement of the silk fibroin scaffolds…

Engineering patient-specific bioprinted constructs for treatment of degenerated intervertebral disc

Materials Today Communications 2019 Volume 19, Pages 506-512

Lower back pain (LBP), which is strongly associated with intervertebral disc (IVD) degeneration, is one of the most frequently reported age- and work-related disorder in actual society, leading to a huge socio-economic impact worldwide. The current treatments have poor clinical outcomes and do not consider each patient needs. Thus, there is a growing interest in the potential of personalized cell-based tissue engineering (TE) approaches aimed to regenerate the damaged IVD and efficiently restore full disc function. In this work, a bioink composed by silk fibroin (SF) hydrogel combined with elastin was used to bioprint patient-specific substitutes mimicking IVD ultrastructure, in…

Fast Setting Silk Fibroin Bioink for Bioprinting of Patient-Specific Memory-Shape Implants

Advanced Healthcare Materials 2017 Volume 6, Issue 22, 1701021

The pursuit for the “perfect” biomimetic and personalized implant for musculoskeletal tissue regeneration remains a big challenge. 3D printing technology that makes use of a novel and promising biomaterials can be part of the solution. In this study, a fast setting enzymatic-crosslinked silk fibroin (SF) bioink for 3D bioprinting is developed. Their properties are fine-tuned and different structures with good resolution, reproducibility, and reliability can be fabricated. Many potential applications exist for the SF bioinks including 3D bioprinted scaffolds and patient-specific implants exhibiting unique characteristics such as good mechanical properties, memory-shape feature, suitable degradation, and tunable pore architecture and morphology.

3D segmentation of intervertebral discs: from concept to the fabrication of patient-specific scaffolds

Journal of 3D Printing in Medicine 2017 Volume 1, Issue 2, Pages 91-101

Aim: To develop a methodology for producing patient-specific scaffolds that mimic the annulus fibrosus (AF) of the human intervertebral disc by means of combining MRI and 3D bioprinting. Methods: In order to obtain the AF 3D model from patient’s volumetric MRI dataset, the RheumaSCORE segmentation software was used. Polycaprolactone scaffolds with three different internal architectures were fabricated by 3D bioprinting, and characterized by microcomputed tomography. Results: The demonstrated methodology of a geometry reconstruction pipeline enabled us to successfully obtain an accurate AF model and 3D print patient-specific scaffolds with different internal architectures. Conclusion: The results guide us toward patient-specific intervertebral…

Building the basis for patient-specific meniscal scaffolds: From human knee MRI to fabrication of 3D printed scaffolds

Bioprinting 2016 Volumes 1–2, Pages 1–10

The current strategies for the transplantation of meniscus should be strengthened to tackle the faced limitations of current methods in the clinics. One of the limitations is that current implants are not patient-specific. There is, therefore, a pressing need in the clinics to develop patient-specific implants. The aim of this study was to demonstrate a semi-automatic way of segmenting meniscus tissues from patients’ volumetric knee magnetic resonance imaging (MRI) datasets in order to obtain patient-specific 3D models for 3D printing of patient-specific constructs. High-quality MRI volumetric images were acquired from five healthy male human subjects. The advanced segmentation software, RheumaSCORE,…