3D Bioplotter Research Papers

Displaying all papers by A. Rajaram (4 results)

Bioprinted fibrin-factor XIII-hyaluronate hydrogel scaffolds with encapsulated Schwann cells and their in vitro characterization for use in nerve regeneration

Bioprinting 2016 Volume 5, March 2017, Pages 1-9

The blood clotting protein fibrin contains cell-binding domains, providing potential advantage for the fabrication of tissue repair scaffolds and for live cell encapsulation. However, fabrication of fibrin scaffolds with encapsulated cells using three dimensional (3D) printing has proven challenging due to the mechanical difficulties of fabricating protein hydrogel scaffolds with defined microstructure. For example, extrusion based 3D printing of fibrin is generally unfeasible because of the low viscosity of precursor fibrinogen solution. Here we describe a novel technique for bioprinting of fibrin scaffolds by extruding fibrinogen solution into thrombin solution, utilizing hyaluronic acid (HA) and polyvinyl alcohol (PVA) to increase…

Use of the polycation polyethyleneimine to improve the physical properties of alginate-hyaluronic acid hydrogel during fabrication of tissue repair scaffolds

Journal of Biomaterials Science, Polymer Edition 2015 Volume 26, Issue 7, Pages 433-445

Recently alginate-based tissue repair scaffolds fabricated using 3D printing techniques have been extensively examined for use in tissue engineering applications. However, their physical and mechanical properties are unfavorable for many tissue engineering applications because these properties are poorly controlled during the fabrication process. Some improvement of alginate gel properties can be realized by addition of hyaluronic acid (HA), and this may also improve the ability of cells to interact with the gel. Here, we report improvement of the physical properties of alginate–HA gel scaffolds by the addition of the polycation polyethyleneimine (PEI) during the fabrication process in order to stabilize…

Bioplotting Alginate/Hyaluronic Acid Hydrogel Scaffolds with Structural Integrity and Preserved Schwann Cell Viability

3D Printing and Additive Manufacturing 2014 Volume 1, Issue 4, Pages 194-203

Bioplotting is an emerging freeform scaffold fabrication technique useful for creating artificial tissue scaffolds containing living cells. Simultaneous maintenance of scaffold structural integrity and cell viability is a challenging task. In this article, we present strategies developed to bioplot alginate-based three-dimensional tissue scaffolds containing hyaluronic acid and living Schwann cells for potential use in peripheral nerve tissue engineering. The fabrication platform, upon which the scaffold is created, was coated with the polycation polyethylenimine to immobilize the first layer of the scaffold on the platform. Each layer was then dispensed into a bath containing calcium chloride to cross-link the alginate, polyvinyl…

Development of Schwann Cell-Encapsulated Alginate Scaffolds for the Repair of Peripheral Nerve Injury

CMBES Proceedings 35 2012

Nerve conduits for peripheral nerve repair have progressed from simple silicon tubes to complex engineered scaffolds. Recent advances in scaffold fabrication have enabled the incorporation of neurotrophins, extracellular matrix components and various cells into scaffolds for enhanced biologic properties. Bioplotting is one of the emerging methods, where the scaffold material, in form of a solution, is dispensed from a needle, layer by layer forming a three-dimensional structure. It enables the use of a wide range of materials, ranging from synthetic polymers (like polycaprolactone, polyglycolic acid, etc.) to naturally occurring polymers like alginate, chitosan, etc. Notably, the use of hydrogels gives…