3D Bioplotter Research Papers

Displaying 10 latest papers (217 papers in the database)

Evaluation of PBS Treatment and PEI Coating Effects on Surface Morphology and Cellular Response of 3D-Printed Alginate Scaffolds

Journal of Functional Biomaterials 2017 Volume 8, Issue 4, Article 48

Three-dimensional (3D) printing is an emerging technology for the fabrication of scaffolds to repair/replace damaged tissue/organs in tissue engineering. This paper presents our study on 3D printed alginate scaffolds treated with phosphate buffered saline (PBS) and polyethyleneimine (PEI) coating and their impacts on the surface morphology and cellular response of the printed scaffolds. In our study, sterile alginate was prepared by means of the freeze-drying method and then, used to prepare the hydrogel for 3D printing into calcium chloride, forming 3D scaffolds. Scaffolds were treated with PBS for a time period of two days and seven days, respectively, and PEI…

Ni-Mn-Ga Micro-trusses via Sintering of 3D-printed Inks Containing Elemental Powders

Acta Materialia 2017

Ni-Mn-Ga magnetic shape memory alloy (SMA) micro-trusses, suitable for high magnetic field induced strains and/or a large magnetocaloric effect, are created via a new additive manufacturing method combining (i) 3D-printing ∼400 μm struts with an ink containing a polymer binder and elemental Ni, Mn, and Ga powders, (ii) binder burn-out and metallic powder interdiffusion and homogenization to create the final alloy, and (iii) further sintering to increase strut density. Controlled amounts of hierarchical porosity, desirable to enable twinning in this polycrystalline alloy, are achieved: (i) continuous ∼450 μm channels between the printed Ni-Mn-Ga ∼300 μm diameter struts (after sintering) and…

3D printing of hybrid biomaterials for bone tissue engineering: Calcium-polyphosphate microparticles encapsulated by polycaprolactone

Acta Biomaterialia 2017

Here we describe the formulation of a morphogenetically active bio-ink consisting of amorphous microparticles (MP) prepared from Ca2+ and the physiological inorganic polymer, polyphosphate (polyP). Those MP had been fortified by mixing with poly-ε-caprolactone (PCL) to allow 3D-bioprinting. The resulting granular PCL/Ca-polyP-MP hybrid material, liquefied by short-time heating to 100 °C, was used for the 3D-printing of tissue-like scaffolds formed by strands with a thickness of 400 µm and a stacked architecture leaving ≈0.5 mm2-sized open holes enabling cell migration. The printed composite scaffold turned out to combine suitable biomechanical properties (Young’s modulus of 1.60 ± 0.1 GPa; Martens hardness of 153 ± 28 MPa), matching those of cortical…

Elastic polyurethane bearing pendant TGF-β1 affinity peptide for potential tissue engineering applications

Materials Science and Engineering: C 2017

Highlights * An elastic degradable polyurethane (PU) bearing pendent HSNGLPL peptide for TGF-β1 affinity binding mimics the extracellular matrix function to retain and release growth factors. * The pendant peptide sequence presented a high affinity for TGF-β1 retaining, even when the surface was pre-coated with other proteins. * The synthesized PU shows good extrusion processing ability and can be printed into 3D scaffolds with designed porous structures. * The released TGF-β1 from surface conjugating was tested by differentiation guiding experiments of ATDC5 cells in vitro and the regeneration of the surrounding tissue after implanting in vivo.

Imaging stem cell distribution, growth, migration, and differentiation in 3-D scaffolds for bone tissue engineering using mesoscopic fluorescence tomography

Biotechnology and Bioengineering 2017

Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2∼3 mm). In this study, we utilized angled fluorescence laminar optical tomography (aFLOT), which allows depth-resolved molecular characterization of engineered tissues in 3-D to investigate cell viability, migration and…

UV-assisted 3D bioprinting of nano-reinforced hybrid cardiac patch for myocardial tissue engineering

Tissue Engineering Part C: Methods 2017

Biofabrication of cell supportive cardiac patches that can be directly implanted on myocardial infarct is a potential solution for myocardial infarction repair. Ideally, cardiac patches should be able to mimic myocardium extracellular matrix for rapid integration with the host tissue, raising the need to develop cardiac constructs with complex features. In particular, cardiac patches should be electrically conductive, mechanically robust and elastic, biologically active and pre-vascularized.. In this study, we aim to biofabricate a nano-reinforced hybrid cardiac patch laden with human coronary artery endothelial cells (HCAECs) with improved electrical, mechanical and biological behavior. A safe UV exposure time with insignificant…

Prevascularization of 3D printed bone scaffolds by bioactive hydrogels and cell co-culture

Journal of Biomedical Materials Research Part B: Applied Biomaterials 2017

Vascularization is a fundamental prerequisite for large bone construct development and remains one of the main challenges of bone tissue engineering. Our current study presents the combination of 3D printing technique with a hydrogel-based prevascularization strategy to generate prevascularized bone constructs. Human adipose derived mesenchymal stem cells (ADMSC) and human umbilical vein endothelial cells (HUVEC) were encapsulated within our bioactive hydrogels, and the effects of culture conditions on in vitro vascularization were determined. We further generated composite constructs by forming 3D printed polycaprolactone/hydroxyapatite scaffolds coated with cell-laden hydrogels and determined how the co-culture affected vascularization and osteogenesis. It was demonstrated…

“Tissue Papers” from Organ-Specific Decellularized Extracellular Matrices

Advanced Functional Materials 2017 Volume 27, Issue 34

Using an innovative, tissue-independent approach to decellularized tissue processing and biomaterial fabrication, the development of a series of “tissue papers” derived from native porcine tissues/organs (heart, kidney, liver, muscle), native bovine tissue/organ (ovary and uterus), and purified bovine Achilles tendon collagen as a control from decellularized extracellular matrix particle ink suspensions cast into molds is described. Each tissue paper type has distinct microstructural characteristics as well as physical and mechanical properties, is capable of absorbing up to 300% of its own weight in liquid, and remains mechanically robust (E = 1–18 MPa) when hydrated; permitting it to be cut, rolled,…

Biomimetic 3D printed scaffolds for meniscus tissue engineering

Bioprinting 2017 Volume 8, Pages 1-7

The menisci distribute loads to protect the articular cartilage of the knee joint from excessive stress. Injuries to their avascular inner regions do not heal, disrupt function, and increase the risk for knee osteoarthritis. Meniscus tissue engineering aims to restore normal meniscus function by use of regenerated tissue on bioengineered scaffolds. The primary purpose of this study was to design and 3D print polycaprolactone scaffolds that recapitulate the shape and structural components of the meniscus extracellular matrix to provide a template and structural support for complete cell-based meniscus regeneration. A secondary aim was to characterize 3D printed polycaprolactone scaffold fibre…

Development and Analysis of a 3D Printed Hydrogel Soft Actuator

Sensors and Actuators A 2017 Volume 265, Pages 94–101

Polyelectrolyte hydrogels produce mechanical motion in response to electrical stimulus making them a good candidate for implementation of soft actuators. However, their customary fabrication process has thus far hindered their applicability in a broad range of controlled folding behaviours. This paper employs the 3D printing technology does the development of polyelectrolyte hydrogel soft actuators. A 3D printed soft hydrogel actuator with contactless electrodes is presented for the first time. Initially chitosan as a candidate of polyelectrolytes which possess both printability and stimuli responsive is opted for ink preparation of 3D printing. The printing parameters are optimised for fabrication of desired…